anti human nos3 rabbit polyclonal antibody  (Santa Cruz Biotechnology)

Journal: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver

Article Title: NOSTRIN is an emerging negative regulator of decompensated cirrhotic patients with portal hypertension.

doi: 10.1016/j.dld.2024.08.050

Figure Lengend Snippet: Fig. 3. (A, i) Pathological changes observed by H&E of human cirrhotic liver and control liver sections (magnification - 10X). The cirrhotic patient’s liver showed increased Nostrin expression and decreased peNOS expression, which was concomitant with reduced NO bioavailability. Representative images (magnification - 20X) of human cirrhotic liver tissue sections showing elevated hepatic Nostrin levels by DAB immunohistochemistry (A, ii). Representative images (magnification - 20X) of human liver tissue sections showing elevated hepatic eNOS levels in the control liver compared to the cirrhotic liver by DAB immunohistochemistry A, iii). Hepatic Nostrin protein expression by immunoblot assay with densitometry quantification (B) and mRNA expression by qPCR (C). Liver total eNOS and p-eNOS expression were evaluated by immunoblot assay with densitometry quantification (D). Hepatic total and phosphorylated eNOS/eNOS in the serine 1177 residue (peNOS) expression by immunoblot assay with densitometry quantification (D) and mRNA expression by qPCR (E). The hepatic cGMP levels were measured by ELISA (F). Data are expressed as mean ± standard error of mean (SEM). Symbols represent ∗p < 0.05; ∗∗p < 0.01 compared to control liver. β-actin was used as an internal loading control.

Article Snippet: Tissues were incubated with 5 % goat s t s ( t o r a H m ( t c s 2 f a a u R t e R A i ( A a w r i P t m 2 t p ( s t ( p R a t ( t i w i a ( H t a 2 t s a s S t l ( P 3 3 a c w t i t s p o B 3 a a erum for 1 hr and incubated overnight at 4 °C with primary anibodies against rabbit polyclonal anti-human Nostrin (1:100; cat: c-134,803, Santacruz) and rabbit polyclonal anti- human eNOS 1:100; cat: PA-1712–1, Bosterbio).

Techniques: Control, Expressing, Immunohistochemistry, Western Blot, Residue, Enzyme-linked Immunosorbent Assay

Journal: Pulmonary Circulation

Article Title: Pulmonary artery endothelium resident endothelial colony-forming cells in pulmonary arterial hypertension

doi: 10.4103/2045-8932.93547

Figure Lengend Snippet: ECFC express endothelial cell-specific markers. Representative images are shown. Both PAEC and ECFC demonstrate positive staining for CD31, vWF, cav-1, eNOS, and CD34. vWF stains intracellularly in both PAEC and ECFC, as expected for endothelial cells. CD31 exhibits staining localized to the membrane, particularly at sites of cell-cell contact, and in the cytosol. The cell-junctional staining for CD31 staining in ECFC is not as pronounced owing to cells not being confluent. eNOS staining is present in both PAEC and ECFC, particularly in the perinuclear region. Cav-1 stains both PAEC and ECFC. There is also CD34 staining in both PAEC and ECFC in both control and PAH groups (arrows). There are no significant differences in staining pattern between PAH and control groups. Scale bar is 50 μm.

Article Snippet: Following aspiration of blocking serum, cells were incubated with 200 μL of the following primary antibodies at the listed dilutions at room temperature for 90 minutes with gentle agitation: polyclonal rabbit anti-human: vWF at 1:1200 (Dako, Glostrup, Denmark); polyclonal rabbit anti-human NOS3 (eNOS) at 1:500 (Santa Cruz Biotechnology, Santa Cruz, Calif.); polyclonal rabbit anti-CD31 prediluted at 1:4 (Abcam, Cambridge, Mass.); monoclonal mouse anti-caveolin 1 at 1:200 (BD Transduction Laboratories, San Jose, Calif.); and monoclonal mouse anti-human CD34 Class II at 1:25 (Dako, Glostrup, Denmark).

Techniques: Staining, Membrane, Control

Journal: Journal of pharmacological sciences

Article Title: Aggravation of cold-restraint stress-induced gastric lesions in adjuvant arthritic rats: pathogenic role of inducible and endothelial nitric oxide.

doi: 10.1254/jphs.09203fp

Figure Lengend Snippet: Fig. 5. Immunohistochemical study for eNOS and iNOS in the gastric mucosa of normal and arthritic rats. The experiment was performed 14 days after the injection of FCA. eNOS and iNOS proteins were stained by Alexa fluor 488 (green) and Alexa Fluor 546 (red), respectively. Immunofluorescence was observed using a confocal laser scanning microscope (LSM-510, magnification: ×200). A: eNOS in normal rats, B: eNOS in arthritic rats, C: iNOS in normal rats, C: iNOS in arthritic rats.

Article Snippet: For the fluorescent immunohistochemical study of eNOS and iNOS, the slides were incubated with rabbit polyclonal anti-human eNOS (NOS 3) antibody (Santa Cruz Biotechnology) or rabbit polyclonal mouse iNOS (NOS 2) antibody (BD Transduction Laboratory) and then treated with Alexa Fluor 488–labeled donkey polyclonal anti-rabbit IgG antibody (Molecular Probes, Eugene, OR, USA) or Alexa Fluor 546–labeled donkey polyclonal anti-rabbit IgG antibody (Molecular Probes), respectively.

Techniques: Immunohistochemical staining, Injection, Staining, Immunofluorescence, Laser-Scanning Microscopy

Journal:

Article Title: Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma *

doi: 10.1631/jzus.2005.B0693

Figure Lengend Snippet: (a) Low grade astrocytoma: Light and few expressions of eNOS in tumor cell, but comparatively strong in the vascular endothelial cells (immunohistochemical staining ×400); (b) Glioblastoma: eNOS strongly expressed in tumor cells (immunohistochemical staining ×400); (c) Glioblastoma: Glomerulus-like eNOS positive vascular structure (immunohistochemical staining ×400); (d) Anaplastic astrocytoma: Median expression of eNOS in tumor cells (immunohistochemical staining ×400); (e) Low grade astrocytoma: Light expression of VEGF in a few tumor cells, but obvious in the vascular endothelial cells (immunohistochemical staining ×400); (f) Anaplastic astrocytoma: Median expression of VEGF in tumor cells (immunohistochemical staining ×400)

Article Snippet: The samples were incubated overnight at 4 °C with rabbit polyclonal antibody against human eNOS (1:50 dilution; Boster Biotechnical Corp., Wuhan, China), mouse polyclonal antibody against human VEGF or mouse monoclonal antibody against human factor VIII related antigen (FVIIIRAg) (1:50 dilution; Zhongshan Biotechnical Corp., Beijing, China).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal:

Article Title: Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma *

doi: 10.1631/jzus.2005.B0693

Figure Lengend Snippet: eNOS-ir, VEGF-ir, MVD in astrocytic hyperplasia and astrocytoma

Article Snippet: The samples were incubated overnight at 4 °C with rabbit polyclonal antibody against human eNOS (1:50 dilution; Boster Biotechnical Corp., Wuhan, China), mouse polyclonal antibody against human VEGF or mouse monoclonal antibody against human factor VIII related antigen (FVIIIRAg) (1:50 dilution; Zhongshan Biotechnical Corp., Beijing, China).

Techniques:

Journal:

Article Title: Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma *

doi: 10.1631/jzus.2005.B0693

Figure Lengend Snippet: The obvious positive correlation of the expression of eNOS and VEGF

Article Snippet: The samples were incubated overnight at 4 °C with rabbit polyclonal antibody against human eNOS (1:50 dilution; Boster Biotechnical Corp., Wuhan, China), mouse polyclonal antibody against human VEGF or mouse monoclonal antibody against human factor VIII related antigen (FVIIIRAg) (1:50 dilution; Zhongshan Biotechnical Corp., Beijing, China).

Techniques: Expressing